Basic Skill Labs
Introduction
The Basic Skill labs conducts studies on small laboratory animals- Mice, rats and rabbits. The facility is a holding facility, no animal breeding is done in this lab. The lab scope is limited to performing inhouse or collaborative studies. The lab is registered with CCSEA (Committee for the control and supervision of experiments in animals). The lab is not a GLP certified lab but we follow good laboratory practises.
Basis Skill Labs
Dr. T. Ranjeet Kumar
PhD
Scientist,
Basic Skill Lab,
Asian Healthcare Foundation
Hyderabad
Biography
Dr. T. Ranjeet Kumar is the Veterinarian and Lab Incharge for both the labs-Basic Skill labs and Skill labs. Dr. Ranjeet is member secretary of IAEC (Institutional Animal Ethics Committee) and responsible for getting study approvals for both small lab and large animal studies. He along with the Department heads and Academic Section Head plans and conducts the large animal training studies in Skill labs. Experimental design and study plans for small lab animal studies are also done by him in collaboration with the PI. He is assisted by Mr. Nagendra and Mr. Mahesh
Areas of Expertise
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Veterinary Medicine and Animal Husbandry
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Microbiology
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Molecular Biology
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Biochemistry
Research
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Chronic pancreatitis, AAH, Pdx, tissue regeneration
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Veterinary General Anaesthesia
Research
Overview
Research Focus Areas
Development of a mice model for Chronic pancreatitis.
Development of a Crohn’s disease-Peri Anal Fistula model in rats
Development of a mice model for Alcohol Associated Hepatitis (AAH)
Patient derived xenografts (Pdx models) in mice
Coordinating large animal training workshops in-Endoscopic procedures, Surgical Gastroenterology, Surgical Gynaecology, Neurosurgery, Regional anaesthesia, Rigid Bronchoscopy
Ongoing Projects / Collaborations
Development of Bio Mimetic Natural Proteinaceous Device for Effective Islet Cell Transplantation to Treat Patients with Diabetes.
To evaluate in vivo biocompatibility and muscle regeneration potential of decellularized esophageal smooth muscle matrix hydrogel for esophageal regeneration in rat and rabbit model.
Inhibition of LPA receptor in L-Arginine induced CP mouse model
Treatment of alcohol associated hepatitis in rodent models
Large animal training programs have been approved by CCSEA in 12 different medical and surgical disciplines. The training schedule is decided by the respective PIs.
Principal Investigator
Dr. T Ranjeet Kumar
Scientist Incharge
Team Members
Mr. V. Nagendra Babu
Mr. P. Mahesh
Modulation of nuclear receptor 4A1 expression improves insulin secretion in a mouse model of chronic pancreatitis. Sheethal G., Verma A., Mall R., Parsa K. V., Tokala R. K., Bynigeri R., Pondugala P. K., Vemula K., Sai Latha S., Sowpati D. T., Singh S. S., Rao G. V., Talukdar R., Kanneganti T. D., Reddy D. N., Sasikala M. Pancreas. 2024; 53(9):e760–e773. doi:10.1097/MPA.0000000000002370.
Dietary green tea extract and antioxidants improve insulin secretory functions of pancreatic β-cells in mild and severe experimental rodent model of chronic pancreatitis. Sheethal G., Tokala R., Pondugala P., Vemula K., Venkatesan V., Suresh P., Singh S., Rao G., Reddy D., Sasikala M. Open Journal of Endocrine and Metabolic Diseases. 2024; 14:53–72. doi:10.4236/ojemd.2024.142007.
Transcriptomic analysis of pancreatic tissue from humans and mice identifies potential gene signatures and unexplored pathways during progression from acute to chronic pancreatitis. Galande S., Ravikanth V. V., Tokala R. K., Satyanarayana Singh S., Rao G. V., Talukdar R., Peddapulla C., Reddy D. N., Sasikala M. Gene. 2025; 940:149200. doi:10.1016/j.gene.2024.149200.
Conference Presentations / Abstracts
Sheethal G, Ravi Kanth V.V., Ranjeet T, Krishna V.V., Mitnala Sasikala, Duvvur Nageshwar R. Transcriptome analysis during progression of chronic pancreatitis identifies sequential receptor alterations.
Gastroenterology. 2019 May; 156(6): S-8.Sheethal G, Ranjeet T, Krishna V.V., Pavan Kumar P, Rao G.V., Duvvuru Nageshwar Reddy, Sasikala M. Green tea extract reverses islet dysfunctions in experimental chronic pancreatitis – Potential therapeutic implications for pancreatogenic diabetes.
Journal of Gastroenterology and Hepatology. 2019; 34(S3): PP0972.Sheethal G, Goudshelwar Renuka, Ranjeet T, Rao G.V., Duvvuru Nageshwar Reddy, Sasikala M. Therapeutic potential of targeting SSR4 in pancreatitis.
Journal of Gastroenterology and Hepatology. 2019; 34(S3): PP0975.Galande S, Kuruva M.M., Ranjeet K.T., Krishna V.V., Mitnala Sasikala, Duvvur Nageshwar R.
Targeting nuclear receptor NR5A2 improves exocrine, endocrine architecture and functions in chronic pancreatitis.
Gastroenterology. 2020 May; 158(6): S-871.Prasanna A, Jakkampudi A, Sarkar P, Tokala R, Sarkar S, Chintaluri S, Unnisa M, Reddy D.N., Talukdar R.
Dysregulated gut barrier function and key microbiome–metabolome alterations is associated with the severity of chronic pancreatitis.
DDW ePoster Library. 2023 May 7; Abstract 379977; Su1887.Raghunathan N, Tokala R, Nagendra B, Pillikala M, Bera A.K., Adhikari J, Reddy H, Lakshmi J.B., Midde S, Singh J, Chavan S, Patel R, Sekaran A, Mitnala S, Pati F, Banerjee R.
Investigating the effect of a novel bio-based tissue glue for perianal fistulas in Crohn’s disease: A low-cost rodent model study.
Journal of Crohn’s and Colitis. 2025 Jan; 19(Suppl 1): i548–i549. Abstract P0155.
